Besifloxacin Hydrochloride (BOL-303224-A) is a fourth-generation fluoroquinolone antibiotic.

Besifloxacin 在针对广泛及耐药病原体方面展现出了有效的杀菌活性。它在对抗革兰阳性病原体和厌氧菌方面被认为是测试中的高效化合物，并且在对抗大多数革兰阴性病原体方面，其活性普遍与其他比较对象的氟喹诺酮相当。Besifloxacin 展示了强大的广谱活性，特别是对于那些对其他氟喹诺酮和不同类别的抗菌剂耐药的革兰阳性和革兰阴性分离物。

20 ng of purified human DDK, together with increasing concentrations of each DDK inhibitor is pre-incubated for 5 min. Then 10 μCi (γ)-32P ATP and 1.5 μM cold ATP are added in a buffer containing 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, and 1 mM DTT and incubated for 30 min at 30°C. The proteins are denatured in 1X Laemmli buffer at 100°C followed by SDS-PAGE and autoradiography on HyBlot CL film. DDK kinase activity is indicated by Auto-phosphorylation of DDK. 32P-labeled bands are quantified using ImageJ and the IC50 values are calculated using GraphPad.